P7643
PIPES dipotassium salt
≥99% (titration)
Synonym(s):
1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Empirical Formula (Hill Notation):
C8H16K2N2O6S2
CAS Number:
Molecular Weight:
378.55
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
Quality Level
assay
≥99% (titration)
form
crystalline powder
useful pH range
6.1-7.5
pKa (25 °C)
6.8
solubility
water: 0.25 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
SMILES string
[K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
InChI key
BJQYFCAMUXGZFN-UHFFFAOYSA-L
General description
PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.
Application
PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.
Still not finding the right product?
Explore all of our products under PIPES dipotassium salt
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation
Nie J, et al.
BMC Developmental Biology, 15(1), 10-10 (2015)
Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin-microtubule complexes
Benoit MPMH and Sosa H
Single Molecule Analysis, 15(1), 199-216 (2018)
Helical alignment inversion of microtubules in accordance with a structural change in their lattice
Shikinaka K, et al.
Soft Matter, 11(19), 3869-3874 (2015)