S5394
Lipoprotein Deficient Serum from fetal calf
frozen liquid
Synonym(s):
Fetal Calf Serum, Lipoprotein Deficient Serum
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About This Item
NACRES:
NA.61
UNSPSC Code:
12352207
Biological source:
bovine fetus
Form:
frozen liquid
Shipped in:
dry ice
Product Name
Lipoprotein Deficient Serum from fetal calf, frozen liquid
biological source
bovine fetus
form
frozen liquid
concentration
≥14 mg protein/mL Biuret
Quality Level
impurities
<1 ng/mg protein endotoxin
<5 % of normal lipoprotein
shipped in
dry ice
storage temp.
−20°C
Application
Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).
General description
Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.
Preparation Note
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5
0.2 μm filtered
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Ying Wang et al.
Cell death discovery, 7(1), 201-201 (2021-08-06)
Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
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