T0825
Anti-Transportin 1 antibody, Mouse monoclonal
clone D45, purified from hybridoma cell culture
Synonym(s):
Anti-IPO2, Anti-KPNB2, Anti-MIP, Anti-MIP1, Anti-TRN
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About This Item
Conjugate:
unconjugated
Clone:
D45, monoclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
microarray
western blot
immunoprecipitation (IP)
microarray
western blot
Species reactivity:
mouse, canine, rat, human, bovine
Citations:
18
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa nuclear extract
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa nuclear extract
Uniprot accession no.:
Product Name
Anti-Transportin 1 antibody, Mouse monoclonal, clone D45, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
D45, monoclonal
form
buffered aqueous solution
mol wt
antigen ~90 kDa
species reactivity
mouse, canine, rat, human, bovine
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa nuclear extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TNPO1(3842)
mouse ... Tnpo1(238799)
rat ... Tnpo1(309126)
Application
Monoclonal Anti-Transportin 1 antibody produced in mouse has been used in western blotting, immunofluorescence, and immunostaining.
Biochem/physiol Actions
Transportin 1 (TNPO1 or TRN1) recognizes the nuclear localization signals (NLS) and binds to the nuclear pore complex (NPC) to facilitate the transport of substances from cytoplasm to nucleus. TRN1 transports viral, and ribosomal proteins with the aid of RanGTP (Ras associated nuclear protein-GTP). TRN1 is also known to regulate the key events in the cell cycle assembly like nuclear membrane and nuclear pore. Mutations in TRN1 might cause amyotrophic lateral sclerosis due to mislocalisation of FUS (fused in sarcoma) protein. TRN1 is associated with regulation of circadian rhythm through nuclear localization of PER1 (period circadian protein homolog 1) protein.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Transportin 1 (TNPO1 or TRN1) also known as karyopherin-β2, is a nuclear import protein and belongs to importin-β family. In the human chromosome the TNPO1 gene is localized on 5q13.2.(10)
Transportin 1 encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes
Immunogen
recombinant human transportin 1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function
Hock EM, et al.
Cell Reports, 24(4), 987-1000 (2018)
Nuclear pore complex protein mediated nuclear localization of dicer protein in human cells
Ando Y, et al.
PLoS ONE, 6(8), e23385-e23385 (2011)
Benjamin Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(15), 8503-8514 (2020-04-03)
The specific interaction of importins with nuclear localization signals (NLSs) of cargo proteins not only mediates nuclear import but also, prevents their aberrant phase separation and stress granule recruitment in the cytoplasm. The importin Transportin-1 (TNPO1) plays a key role
The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization
Korge S, et al.
PLoS Genetics, 14(1), e1007189-e1007189 (2018)
M E Cicardi et al.
Communications biology, 7(1), 376-376 (2024-03-29)
Expanded intronic G4C2 repeats in the C9ORF72 gene cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). These intronic repeats are translated through a non-AUG-dependent mechanism into five different dipeptide repeat proteins (DPRs), including poly-glycine-arginine (GR), which is aggregation-prone and
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