AB3553

Anti-Thiazide-Sensitive NaCl Cotransporter Antibody

Name: Anti-Thiazide-Sensitive NaCl Cotransporter Antibody
Brand: MM

Chemicon^®, from rabbit

Synonym(s):

Anti-NCC, Anti-NCCT, Anti-TSC

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Thiazide-Sensitive NaCl Cotransporter Antibody, Chemicon^®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rabbit, mouse, rat, human

manufacturer/tradename

Chemicon^®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000339.1

UniProt accession no.

P55017

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SLC12A3(6559)

Analysis Note

Control
POSITIVE CONTROL: Rat kidney.

Application

Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended.

Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.

Biochem/physiol Actions

Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

160-190 kDa

Immunogen

Synthetic peptide from the N-terminal of human/rat/mouse NCC.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB3564P

Physical form

Affinity Purified immunoglobulin in PBS containing no preservatives.

ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genomic-derived markers for early detection of calcineurin inhibitor immunosuppressant-mediated nephrotoxicity.

Cui, Y; Huang, Q; Auman, JT; Knight, B; Jin, X; Blanchard, KT; Chou, J; Jayadev, S; Paules, RS

Toxicological Sciences null

Tubule-specific ablation of endogenous β-catenin aggravates acute kidney injury in mice.

Dong Zhou et al.

Kidney international, 82(5), 537-547 (2012-05-25)

β-Catenin is a unique intracellular protein functioning as an integral component of the cell-cell adherens complex and a principal signaling protein mediating canonical Wnt signaling. Little is known about its function in adult kidneys in the normal physiologic state or

Glucocorticoids Induce Nondipping Blood Pressure by Activating the Thiazide-Sensitive Cotransporter.

Jessica R Ivy et al.

Hypertension (Dallas, Tex. : 1979), 67(5), 1029-1037 (2016-03-10)

Blood pressure (BP) normally dips during sleep, and nondipping increases cardiovascular risk. Hydrochlorothiazide restores the dipping BP profile in nondipping patients, suggesting that the NaCl cotransporter, NCC, is an important determinant of daily BP variation. NCC activity in cells is

Renal tubule angiotensin II type 1 receptor-associated protein promotes natriuresis and inhibits salt-sensitive blood pressure elevation.

Wakui, H; Uneda, K; Tamura, K; Ohsawa, M; Azushima, K; Kobayashi, R; Ohki, K; Dejima et al.

Journal of the American Heart Association null

Activation of hepatocyte growth factor receptor, c-met, in renal tubules is required for renoprotection after acute kidney injury.