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Merck

19362

4-Methylumbelliferyl butyrate

lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence

Synonym(s):

MU-Bu

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About This Item

Empirical Formula (Hill Notation):
C14H14O4
CAS Number:
17695-46-4
Molecular Weight:
246.26
NACRES:
NA.32
PubChem Substance ID:
57647789
UNSPSC Code:
12352204
EC Number:
241-694-7
MDL number:
MFCD00038087
Beilstein/REAXYS Number:
227973

Key Documents

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Product Name

4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)

Quality Level

100

assay

≥95% (HPCE)

form

powder or crystals

mp

90-92 °C (lit.)

solubility

DMF: soluble, DMSO: soluble

fluorescence

λex 312 nm in methanol, λex 360 nm; λem 449 nm (Reaction product), λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

InChI key

WKPUJZVCZXWKCK-UHFFFAOYSA-N

Application

suitable as fluorogenic substrate for esterases/lipases

Other Notes

Fluorogenic substrate for butyrate esterase

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9202
BCCH3821
BCCJ6994
BCCC8692
BCBX4197

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M Vaneechoutte et al.
Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5
Ivan Kurtovic et al.
Fish physiology and biochemistry, 36(4), 1041-1060 (2010-02-10)
Lipases were purified from delipidated pyloric ceca powder of two New Zealand-sourced fish, Chinook salmon (Oncorhynchus tshawytscha) and hoki (Macruronus novaezelandiae), by fractional precipitation with polyethylene glycol 1000, followed by affinity chromatography using cholate-Affi-Gel 102, and gel filtration on Sephacryl
Neda Gilannejad et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-24)
Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of
Determination of lipase activity using image analysis.
Marcelo Müller-Santos et al.
Analytical biochemistry, 351(2), 305-307 (2006-03-10)
E Speeleveld et al.
Journal of clinical microbiology, 32(5), 1362-1363 (1994-05-01)
Moraxella catarrhalis can easily be differentiated from other oxidase-positive, gram-negative cocci with tributyrine, 4-methylumbelliferyl butyrate, or indoxyl acetate. All M. catarrhalis give positive reactions, and all Neisseria spp. give negative reactions. The 4-methylumbelliferyl butyrate tube test and indoxyl acetate strip
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