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Merck

67060

Methyl Green

zinc chloride salt, suitable for microscopy

Synonym(s):

Ethyl Green

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About This Item

Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
EC Number:
230-415-4
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329760277
MDL number:
MFCD00151094
Colour Index Number:
42590
Beilstein/REAXYS Number:
3914106

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Product Name

Methyl Green, zinc chloride salt, suitable for microscopy

Quality Level

200

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

form

powder

technique(s)

titration: suitable

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥900 at 624-634 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Analysis Note

Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts.

Application

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Biochem/physiol Actions

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP1291
BCCN9152
BCCN7083
BCCN5376
BCCN2738

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K R Fox et al.
European journal of histochemistry : EJH, 36(3), 263-270 (1992-01-01)
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At
Evaluation of apoptosis as a mechanism of follicular cell atresia in the ovaries of cattle (bos indicus) and buffalo (bubalus bubalis) fetuses.
Santos SSD, et al.
Animal Reproduction, 10, 55-61 (2013)
Monocyte chemoattractant protein-1 in human atheromatous plaques.
Nelken NA, et al.
The Journal of Clinical Investigation, 88, 1121-1121 (1991)
Paxton M Moon et al.
Journal of molecular medicine (Berlin, Germany), 93(8), 845-856 (2015-07-04)
Osteoarthritis (OA) is a highly prevalent, disabling joint disease with no existing therapies to slow or halt its progression. Cartilage degeneration hallmarks OA pathogenesis, and pannexin 3 (Panx3), a member of a novel family of channel proteins, is upregulated during
Kazuhiro Shirozu et al.
Shock (Augusta, Ga.), 42(6), 570-577 (2014-07-22)
Acute liver failure (ALF) is a fatal syndrome associated with massive hepatocyte death. There is no cure for ALF except liver transplantation. Protein farnesylation is a lipid modification of cysteine residues that is catalyzed by farnesyltransferase (FTase) and has been
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