83702
Rhodamine 123
BioReagent, suitable for fluorescence, ≥85% (HPLC)
Synonym(s):
2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
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About This Item
Linear Formula:
C21H17ClN2O3
CAS Number:
Molecular Weight:
380.82
UNSPSC Code:
12352116
NACRES:
NA.32
PubChem Substance ID:
EC Number:
263-687-8
Beilstein/REAXYS Number:
6030951
MDL number:
product line
BioReagent
Quality Level
assay
≥85% (HPLC)
form
powder
impurities
≤10% water
solubility
ethanol: 20 mg/mL, clear, red
suitability
in accordance for fluorescence, suitable for fluorescence
SMILES string
Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
InChI
1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
InChI key
MYFATKRONKHHQL-UHFFFAOYSA-N
General description
Rhodamine 123 is a fluorescent dye.
Application
Rhodamine 123 has been used:
- as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
- to label the cells for estimating the P-gp expression by flow cytometry
- in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
- the detection of mitochondrial membrane potential (ΔΨm)
Biochem/physiol Actions
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Other Notes
Supravital mitochondrial-specific dye
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Wu D, et al.
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Nuclear medicine and biology, 39(8), 1219-1225 (2012-09-18)
With the aim to develop a PET tracer to visualize P-glycoprotein (Pgp) expression levels in different organs, the Pgp inhibitor MC113 was labeled with (11)C and evaluated using small-animal PET. [(11)C]MC113 was synthesized by reaction of O-desmethyl MC113 with [(11)C]methyl