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About This Item
Biological source:
bovine
Assay:
90-120 mg/mL protein basis (biuret)
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤0.05% fatty acid, ≤200 EU/mL endotoxin
Concentration:
10% in DPBS
biological source
bovine
sterility
sterile-filtered
product line
BioReagent
assay
90-120 mg/mL protein basis (biuret)
form
solution
purified by
heat shock fractionation
contains
does not contain stabilizer or preservative
origin
USA origin
concentration
10% in DPBS
technique(s)
cell culture | mammalian: suitable
impurities
≤0.05% fatty acid, ≤200 EU/mL endotoxin
pH
7.1-7.7
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ALB(280717)
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Application
Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.
BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.
BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.
Features and Benefits
- Fatty acid free / tested to be low in fatty acid content
- Low endotoxin
- Sterile-filtered
Preparation Note
Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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