A2095
Monoclonal Anti-HA−Agarose antibody produced in mouse
clone HA-7, purified immunoglobulin, PBS suspension
Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
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About This Item
Conjugate:
agarose conjugate
Clone:
HA-7, monoclonal
Application:
—
Citations:
621
biological source
mouse
Quality Level
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
PBS suspension
isotype
IgG1
capacity
20-50 nmol/mL elution capacity (HA-tagged fusion protein), 30-50 nmol/mL binding capacity (HA-tagged fusion protein)
shipped in
wet ice
storage temp.
2-8°C
General description
The HA tag is a 9-amino acid tag, with the sequence YPYDVPDYA (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), derived from the hemagglutinin (HA) protein at amino acid residues 98-106 in the HA sequence. The HA tag contains a high proportion of charged amino acid residues, which makes the HA tag likely to form a strong antibody recognition site. Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured/reduced forms of HA-tagged proteins. This product is reactive with N-or C-terminal HA-tagged fusion proteins that are expressed in E. coli or in mammalian cells.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Suitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Physical form
Suspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azide
Preparation Note
Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0 - 2.4 mg/mL settled resin.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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