B9673
1-Bromo-3-chloropropane
for isolation of RNA
Synonym(s):
1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Linear Formula:
Cl(CH2)3Br
CAS Number:
Molecular Weight:
157.44
UNSPSC Code:
41105515
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-697-1
Beilstein/REAXYS Number:
605278
MDL number:
Assay:
≥98.5% (GC)
Grade:
Molecular Biology
Bp:
144-145 °C (lit.)
grade
Molecular Biology
Quality Level
assay
≥98.5% (GC)
form
liquid
refractive index
n20/D 1.486 (lit.)
bp
144-145 °C (lit.)
density
1.592 g/mL at 25 °C (lit.)
storage temp.
room temp
SMILES string
ClCCCBr
InChI
1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
InChI key
MFESCIUQSIBMSM-UHFFFAOYSA-N
General description
BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.
Application
1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
- feline teeth
- mice brain and liver tissues
- human ectocervical epithelial cells
- placental samples
- human mesenchymal stromal cells (hMSCs)
Features and Benefits
- less toxic alternative to chloroform
- compatible with TRI reagent for isolation of RNA with decreased DNA contamination
Other Notes
BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.
Still not finding the right product?
Explore all of our products under 1-Bromo-3-chloropropane
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation.
P Chomczynski et al.
Analytical biochemistry, 225(1), 163-164 (1995-02-10)
Ghalia Boubaker et al.
PLoS neglected tropical diseases, 14(5), e0007640-e0007640 (2020-05-23)
We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection of experimental primary alveolar echinococcosis (AE), a parasitic infection caused upon ingestion of E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice
Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins
Bischoff SR, et al.
Testing, 8(1), e55345-e55345 (2013)