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I8896

IGEPAL® CA-630

Molecular Biology

Synonym(s):

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
9002-93-1
UNSPSC Code:
12352104
NACRES:
NA.31
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Quality Level

200

grade

Molecular Biology

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

viscosity

~250-350 cP(25 °C)

mp

6 °C

solubility

water: soluble at 20 °C

density

1.060 g/cm3

HLB

13

foreign activity

DNase and RNase, none detected

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

Analysis Note

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Other Notes

Nonionic, non-denaturing detergent

Legal Information

IGEPAL is a registered trademark of Solvay

Disclaimer

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

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signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ9158
MKCZ6517
MKCZ1618
MKCX2388
MKCW7585

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Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
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