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M1255

Calcein Blue

Powder

Synonym(s):

4-Methylumbelliferone-8-methyliminodiacetic acid

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About This Item

Empirical Formula (Hill Notation):
C15H15NO7
CAS Number:
54375-47-2
Molecular Weight:
321.28
NACRES:
NA.47
PubChem Substance ID:
24896629
UNSPSC Code:
12171500
EC Number:
259-129-8
MDL number:
MFCD00006867
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Product Name

Calcein Blue, fluorescent dye

Quality Level

200

form

powder

technique(s)

titration: suitable

color

white to light yellow

solubility

1 M NaOH: 50 mg/mL

fluorescence

λex 322 nm; λem 445 nm in methanol

suitability

suitable for indicator for EDTA titration

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12

InChI

1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)

InChI key

RJPSHDMGSVVHFA-UHFFFAOYSA-N

General description

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Application

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000444922
0000444922
0000377014
0000353433
0000326239

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Xiaomian Wu et al.
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Compared with titanium (Ti) and other metal implant materials, poly(ether-ether ketone) (PEEK) shows outstanding biomechanical properties. A number of studies have also reported attractive bioactivity for nano-TiO(2) (n-TiO(2)). In this study, n-TiO(2)/PEEK nanocomposites were prepared, taking advantage of the unique
Liangbo Hu et al.
Virologica Sinica, 34(4), 454-466 (2019-06-16)
Baculovirus can transduce a wide range of mammalian cells and is considered a promising gene therapy vector. However, the low transduction efficiency of baculovirus into many mammalian cells limits its practical application. Co-expressing heterologous viral glycoproteins (GPs), such as vesicular
T Lymphocytes Influence the Mineralization Process of Bone.
El Khassawna T
Frontiers in Immunology, 8, 562-562 (2017)
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