Skip to Content
Merck

M6900

Methyl Blue

powder or crystals

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
28983-56-4
Molecular Weight:
799.80
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329818037
EC Number:
249-352-9
Colour Index Number:
42780
MDL number:
MFCD00058509

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

powder or crystals

Quality Level

200

mp

>-23 °C

solubility

H2O: 1 mg/mL, blue

εmax

≥650 at 594-610  nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin

Biochem/physiol Actions

Methyl Blue is considered as a xenobiotic compound and has antiseptic property. It is useful in polychrome staining. Methyl blue is mostly used for staining samples in histology and microbiology. It is also useful as a model to reveal the role of catalyst in light induced degradation of dyes.

wgk

WGK 2

flash_point_f

>572.0 °F - Pensky-Martens closed cup

flash_point_c

> 300 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

Storage Class

11 - Combustible Solids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBS7164
SHBR6766
SHBN9183
SHBL9686
MKCJ1341

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A new principle in polychrome staining: a system of automated staining, complementary to hematoxylin and eosin, and usable as a research tool
Shoobridge Michael PK
Stain Technology, 58(5), 245-258 (1983)
Micellar effect on the kinetics of oxidation of methyl blue by Ce (IV) in sulfuric acid medium
Hassan M, et al.
Arabian Journal of Chemistry, 8(1), 72-77 (2015)
A novel alpha-1, 3-glucan elicits plant defense responses in potato and induces protection against Rhizoctonia solani AG-3 and Fusarium solani f. sp. eumartii
Wolski EA, et al.
Physiological and Molecular Plant Pathology, 69(1-3), 93-103 (2006)
Replication stress in early S phase generates apparent micronuclei and chromosome rearrangement in fission yeast
Sabatinos SA, et al.
Molecular Biology of the Cell, 26(19), 3439-3450 (2015)
A novel a-1, 3-glucan elicits plant defense responses in potato and induces protection against Rhizoctonia solani AG-3 and Fusarium solani f. sp. eumartii.
Wolski E
Physiological & Molecular Plant Pathology, 69, 93-93 (2006)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service