PLA0071
Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified
Powered by Bethyl Laboratories, Inc.
Synonym(s):
32kDa, REPA2, RF-A protein 2, RP-A p32, RP-A p34, RPA32, replication factor A protein 2, replication protein A 34 kDa subunit, replication protein A2, replication protein A2 (32kD)
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Species reactivity:
human, mouse
Technique(s):
immunocytochemistry: 1:500-1:5,000, immunohistochemistry: 1:500-1:2,000, immunoprecipitation (IP): 2-10 μg/mg, western blot: 1:2,000-1:10,000
Application:
ICC, IHC, IP, WB
Citations:
5
biological source
rabbit
Quality Segment
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
species reactivity
human, mouse
technique(s)
immunocytochemistry: 1:500-1:5,000, immunohistochemistry: 1:500-1:2,000, immunoprecipitation (IP): 2-10 μg/mg, western blot: 1:2,000-1:10,000
accession no.
NP_002937.1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer4/pSer8)
Gene Information
human ... RPA2(6118)
General description
Replication protein A2 (RPA32) is encoded by the gene mapped to human chromosome 1p35.3. It is a subunit of the heterotrimeric replication protein A (RPA) complex.
Immunogen
Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).
Biochem/physiol Actions
Replication Protein A (RPA) binds single-stranded DNA and plays a vital role in DNA replication, DNA repair, and checkpoint signaling. Phosphorylation of replication protein A2 (RPA32) by ataxia telangiectasia and Rad3-related protein kinase (ATR) and DNA-dependent protein kinase (DNA-PK) causes checkpoint kinase 1 (Chk1) activation and replication arrest. DNA-PK and ATR signaling via RPA32 (S4/S8) enhances genome stability and cell survival following replication stress.
Physical form
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Other Notes
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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