MAB3402-AF647
Anti-GFAP, clone GA5, AF™ 647 Conjugate
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 647 conjugate
Clone:
GA5, monoclonal
Application:
ICC, IHC (p), WB
Citations:
-
Quality Segment
biological source
mouse
conjugate
ALEXA FLUOR™ 647 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GA5, monoclonal
species reactivity
rat, pig, bovine, human, mouse, rabbit, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
50 kDa
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Immunogen
Purified Glial Fibrillary Acidic Protein (GFAP) from pig spinal cord (Debus, E., 1983).
Application
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.
Immunocytochemistry:
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting: Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting: Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Biochem/physiol Actions
Expected to cross-react with porcine, chicken, bovine, and rabbit.
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
Physical form
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Preparation Note
Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.
DO NOT FREEZE.
Analysis Note
Control
Mouse brain tissue, Astrocyte culture.
Mouse brain tissue, Astrocyte culture.
Routinely evaluated by Western Blot on Mouse brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary
Acidic Protein on 10 μg of Mouse Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary
Acidic Protein on 10 μg of Mouse Brain lysate.
Other Notes
Replaces: 04-1031; 04-1062
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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