Guanidine hydrochloride

Name: Guanidine hydrochloride
Brand: SIGMA

BioUltra, Molecular Biology, ≥99.5% (AT)

Synonym(s):

Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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About This Item

Linear Formula:

NH₂C(=NH)NH₂ · HCl

CAS Number:

50-01-1

Molecular Weight:

95.53

UNSPSC Code:

12352107

NACRES:

NA.28

PubChem Substance ID:

57651284

EC Number:

200-002-3

Beilstein/REAXYS Number:

3591990

MDL number:

MFCD00013026

Product Name

Guanidine hydrochloride, BioUltra, Molecular Biology, ≥99.5% (AT)

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

SMILES string

Cl[H].NC(N)=N

biological source

synthetic

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (AT)

form

powder or crystals

technique(s)

RNA extraction: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.05% (as SO₄)

loss

≤0.05% loss on drying, 20 °C (HV)

color

colorless to white,

pH

4.5-6.0 (25 °C, 6 M in H₂O)

mp

180-185 °C (lit.)

solubility

H₂O: 6 M at 20 °C, clear, colorless

density

1.3 g/cm³ (lit.)

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤150 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

6 M in H₂O

UV absorption

λ: 260 nm A_max: 0.03
λ: 280 nm A_max: 0.02

storage temp.

room temp

Quality Level

200

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Related Categories

Biochemicals

Detergents - Anionic, Cationic, Zwitterionic, Anti-foaming

High-Purity Biochemicals and Reagents

Application

Guanidine hydrochloride (GdnHCl) has been used as a component of the phosphate buffer saline (PBS) for the incubation of Schlemm′s canal (SC) cells. It has also been used as a component of the digestion buffer for the gelation and expansion of human HeLa229 cells. GdnHCl is also used to isolate RNA.

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GdnHCl) is involved in conformational changes and loss of enzyme activity due to inactivation of the enzyme. It is involved in the loss of activity of alkaline phosphatase (ALPase) enzyme in Haliotis diversicolor. GdnHCl functions by hampering the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It can also inactivate enzymes like papain and aminocyclase. Higher concentrations of GdnHCl leads to viral inactivation of herpes simplex virus 1 (HSV-1).

General description

Guanidine hydrochloride (GdnHCl) is a well-known protein and enzyme denaturant. It is a small molecule and hydroscopic in nature.

pictograms

GHS07

signalword

Warning

hcodes

H302 + H332,H315,H319

pcodes

P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000487184

BCCN6783

0000433845

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Use of Protein Folding Reagents.

Current protocols in protein science, 84, A-A (2016-04-03)

The reagents and methods for purification and use of the most commonly used denaturants, guanidine hydrochloride (guanidine-HCl) and urea, are described. Other protein denaturants and reagents used to fold proteins are briefly mentioned. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or

Unfolding and inactivation of abalone (Haliotis diversicolor) alkaline phosphatase during denaturation by guanidine hydrochloride.

Jin Hua Liao et al.

Applied biochemistry and biotechnology, 158(2), 323-333 (2008-10-22)

Abalone, a kind of low poikilothermic invertebrate, is easily exposed to ocean environment stress. Since it is one of the important mariculture animals, the attention paid to the abalone study becomes increasing. Alkaline phosphatase (ALPase, EC 3.1.3.1) is a kind

Arginine-enveloped virus inactivation and potential mechanisms.

Christa Meingast et al.

Biotechnology progress, 36(2), e2931-e2931 (2019-10-18)

Arginine synergistically inactivates enveloped viruses at a pH or temperature that does little harm to proteins, making it a desired process for therapeutic protein manufacturing. However, the mechanisms and optimal conditions for inactivation are not fully understood, and therefore, arginine

Isolation of RNA using guanidinium salts.

R J MacDonald et al.

Methods in enzymology, 152, 219-227 (1987-01-01)

Detection of Chlamydia Developmental Forms and Secreted Effectors by Expansion Microscopy.

Tobias C Kunz et al.

Frontiers in cellular and infection microbiology, 9, 276-276 (2019-08-27)

Expansion microscopy (ExM) is a novel tool to improve the resolution of fluorescence-based microscopy that has not yet been used to visualize intracellular pathogens. Here we show the expansion of the intracellular pathogen Chlamydia trachomatis, enabling to differentiate its two

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