A2228

Anti-β-Actin (ACTB) Antibody

Name: Anti-β-Actin (ACTB) Antibody
Brand: SIGMA

mouse monoclonal, AC-74

Synonym(s):

Actin Antibody Sigma, Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse, Beta Actin Antibody Sigma, Beta Actin Sigma Antibody, Sigma Actin Antibody, Sigma Beta Actin Antibody

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164531

Product Name

Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-74, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-74, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human

should not react with

Dictyostelium discoideum

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts
immunohistochemistry: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts

isotype

IgG2a

UniProt accession no.

P60709

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

300

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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Application

Monoclonal Anti-β-Actin antibody has been used in western blot and two-dimensional gel immunoblot.

Monoclonal anti-β-actin antibody can be used for immunocytochemistry (10-40μg/mL using human or chicken fibroblasts), immunohistochemistry and microarray analysis.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.

Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000447531

0000430827

0000381524

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Immune Pathology Associated with Altered Actin Cytoskeleton Regulation.

Dilki W, et al.

Autoimmunity, 43(1), 64-75 (2010)

β-Actin specifically controls cell growth, migration, and the G-actin pool

Tina M B, et al.

Molecular Biology of the Cell, 22(21) (2011)

Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells

Alexandra CS, et al.

Cancer Letters, 276(1), 32-37 (2009)

The chromatin remodeling protein BRG1 links ELOVL3 trans-activation to prostate cancer metastasis.

Yuyu Yang et al.

Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(8), 834-845 (2019-06-04)

Prostate cancer malignancies are intimately correlated with deregulated fatty acid metabolism. The underlying epigenetic mechanism is not fully understood. In the present study we investigated the mechanism whereby the chromatin remodeling protein BRG1 regulates the transcription of long-chain fatty acid

The protein phosphatase isoform PP1γ1 substitutes for PP1γ2 to support spermatogenesis but not normal sperm function and fertility†.

Tejasvi Dudiki et al.

Biology of reproduction, 100(3), 721-736 (2018-11-01)

Four isoforms of serine/threonine phosphatase type I, PP1α, PP1β, PP1γ1, and PP1γ2, are derived from three genes. The PP1γ1 and PP1γ2 isoforms are alternately spliced transcripts of the protein phosphatase 1 catalytic subunit gamma gene (Ppp1cc). While PP1γ1 is ubiquitous

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