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About This Item
Empirical Formula (Hill Notation):
C26H40O
CAS Number:
Molecular Weight:
368.60
MDL number:
NACRES:
NA.25
UNSPSC Code:
12352211
Product Name
alkyne-cholesterol, 27-norcholest-5-en-25-yn-3β-ol, powder
assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 100 μg (700146P-100ug), pkg of 1 × 250 μg (700146P-250ug)
manufacturer/tradename
Avanti Research™ - A Croda Brand 700146P
lipid type
click reagents
shipped in
dry ice
storage temp.
−20°C
General description
This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry′, in the presence of a copper (Cu)-containing catalyst.
Alkyne-cholesterol is a clickable cholesterol probe proprietary for Avanti Polar Lipids. They differ from cholesterol by having an alkyne group.
Biochem/physiol Actions
Alkyne-cholesterol is not applicable for use in living cells due to copper ion (Cu (I) and Cu (II)) requirements. It aids in easy and sensitive detection of cholesterol using various biophysical methods. Alkyne-cholesterol can be conjugated to boron-dipyrromethene (BODIPY) fluorescent dye. It is useful in cholesterol trafficking studies in various organelles including mitochondria, endoplasmic reticulum and Golgi.
Packaging
5 mL Amber Glass Screw Cap Vial (700146P-100ug)
5 mL Amber Glass Screw Cap Vial (700146P-250ug)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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J Králová et al.
Folia biologica, 65(1), 1-10 (2019-06-07)
Cellular cholesterol plays fundamental and diverse roles in many biological processes and affects the pathology of various diseases. Comprehensive and detailed understanding of the cellular functions and characteristics of cholesterol requires visualization of its subcellular distribution, which can be achieved
Cholesterol: Revisiting its fluorescent journey on 200th anniversary of Chevruel?s ?cholesterine?
Chaudhuri A and Anand D
Biomedical Spectroscopy and Imaging , 6(1-2), 1/24/2020-1/24/2020 (2017)
Katarzyna A Solanko et al.
Lipid insights, 8(Suppl 1), 95-114 (2015-01-01)
Cholesterol transport between cellular organelles comprised vesicular trafficking and nonvesicular exchange; these processes are often studied by quantitative fluorescence microscopy. A major challenge for using this approach is producing analogs of cholesterol with suitable brightness and structural and chemical properties