OP44
Anti-APC (Ab-1) Mouse mAb (FE9)
liquid, clone FE9, Calbiochem®
Synonym(s):
Anti-Adenomatous Polyposis Coli
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
FE9, monoclonal
Species reactivity:
rat, human, mouse
Application:
—
Citations:
23
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
FE9, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (1 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... APC(324)
General description
Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.
- Antibody Target Gene Symbol: APC
- Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
- Entrez Gene Name: adenomatous polyposis coli
- Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L)
- Mu Entrez ID: 11789
- Rat Entrez ID: 24205
Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.
Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.
Immunogen
a synthetic peptide corresponding to the N-terminal 35 amino acids of APC
Application
Immunoblotting (1 µg/ml, see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.
Analysis Note
Positive Control
HCT116 cells for p300, SW480 cells for truncated APC (p147)
HCT116 cells for p300, SW480 cells for truncated APC (p147)
Other Notes
Koetsier, P. A., et al. 1993. BioTechniques15, 258.
Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
Su, L.-K., et al. 1993. Can. Res.53, 2728.
Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
D′Amico, D., et al. 1992. Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
Powell, S. M., et al. 1992. Nature359, 235.
Groden, J., et al. 1991. Cell66, 589.
Kinzler, K. W., et al. 1991. Science253, 661.
Nishisho, I., et al. 1991. Science253, 665.
Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
Su, L.-K., et al. 1993. Can. Res.53, 2728.
Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
D′Amico, D., et al. 1992. Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
Powell, S. M., et al. 1992. Nature359, 235.
Groden, J., et al. 1991. Cell66, 589.
Kinzler, K. W., et al. 1991. Science253, 661.
Nishisho, I., et al. 1991. Science253, 665.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Tamar Evron et al.
Oncogenesis, 10(9), 63-63 (2021-09-24)
The Wnt signaling pathways play fundamental roles during both development and adult homeostasis. Aberrant activation of the canonical Wnt signal transduction pathway is involved in many diseases including cancer, and is especially implicated in the development and progression of colorectal
Mireia Menéndez et al.
Gastroenterology, 134(1), 56-64 (2008-01-02)
We identified the APC N1026S variant of unknown malignant potential in the adenomatous polyposis coli (APC) gene in a Spanish attenuated familial adenomatous polyposis (AFAP) family. The variant was located in the first of the 4 highly conserved 15-amino acid
Hideaki Toki et al.
Cancer science, 104(7), 937-944 (2013-04-05)
Mutant mouse models are indispensable tools for clarifying the functions of genes and elucidating the underlying pathogenic mechanisms of human diseases. We carried out large-scale mutagenesis using the chemical mutagen N-ethyl-N-nitrosourea. One specific aim of our mutagenesis project was to