A1603
Alginate Lyase
powder, ≥10,000 units/g solid
Synonym(s):
poly-beta-D-mannuronate lyase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Product Name
Alginate Lyase, powder, ≥10,000 units/g solid
form
powder
specific activity
≥10,000 units/g solid
storage temp.
2-8°C
Quality Level
Application
Alginate Lyase has been used:
- in the pre-processing of enzyme
- to de-encapsulate cells
- to digest Pseudomonas exopolysaccharide to facilitate electroporation
Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .
Biochem/physiol Actions
Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.
Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.
General description
Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.
Other Notes
One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Preparation Note
Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Yuya Morimoto et al.
APL bioengineering, 4(2), 026101-026101 (2020-04-09)
Biohybrid robots composed of biological and synthetic components have been introduced to reconstruct biological functions in mechanical systems and obtain better understanding of biological designs. For example, biohybrid robots powered by skeletal muscle tissue have already succeeded in performing various
Solaleh Khoramian Tusi et al.
Biomaterials, 32(23), 5438-5458 (2011-05-06)
Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. We sought to elucidate possible effects of alginate oligosaccharide (AOS) on H(2)O(2)-induced cell death and to determine the underlying molecular mechanisms in neuron-like PC12 cells. We found
Lena C E Lundqvist et al.
Carbohydrate research, 352, 44-50 (2012-03-21)
The gene coding for an alginate lyase from the marine bacteria Pseudomonas alginovora X017 was cloned and heterologously expressed in Escherichia coli strains. The protein was produced in inclusion bodies and the active form was obtained by applying a refolding
Bacteriophages phi MR299-2 and phi NH-4 Can Eliminate Pseudomonas aeruginosa in the Murine Lung and on Cystic Fibrosis Lung Airway Cells
Alemayehu D, et al.
mBio, 3(2) (2012)
Elizabeth M Parrish et al.
Reproduction (Cambridge, England), 142(2), 309-318 (2011-05-26)
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.
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