C1655
Cholera Toxin B subunit
GM1-binding, lyophilized powder
Synonym(s):
CTB
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About This Item
Form:
lyophilized powder
Quality level:
Product Name
Cholera Toxin B subunit, FITC conjugate, lyophilized powder
conjugate
FITC conjugate
Quality Level
form
lyophilized powder
mol wt
~12 kDa
composition
Protein, ~20% Lowry
extent of labeling
~1.0 mol FITC per mol protein
storage temp.
2-8°C
SMILES string
CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
InChI
1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
InChI key
YDXZSNHARVUYNM-UHFFFAOYSA-N
Application
Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
- to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat
- to stain epididymal sperms of mice
- to stain peritoneal macrophages of mice
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Analysis Note
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Laura Aragoneses-Fenoll et al.
Journal of leukocyte biology (2021-02-03)
The interaction between the T-lymphocyte costimulatory molecule ICOS and its ligand (ICOS-L) is needed for efficient immune responses, but expression levels are tightly controlled, as altered expression of ICOS or ICOS-L may lead to immunodeficiency, or favor autoimmune diseases and
Lindsey C Felix et al.
Aquatic toxicology (Amsterdam, Netherlands), 192, 58-68 (2017-09-18)
The ever-growing production of engineered nanoparticles (NPs) for use in many agricultural, commercial, consumer, and industrial applications will lead to their accidental or intentional release into the environment. Potential routes of environmental exposure include manufacturing or transport spills, disposal of
Jill M Steinbach et al.
Acta biomaterialia, 30, 49-61 (2015-11-26)
The surface modification of nanoparticles (NPs) can enhance the intracellular delivery of drugs, proteins, and genetic agents. Here we studied the effect of different surface ligands, including cell penetrating peptides (CPPs), on the cell binding and internalization of poly(lactic-co-glycolic) (PLGA)