C5024
Carbonic Anhydrase from bovine erythrocytes
non-denaturing PAGE marker
Synonym(s):
Carbonate Dehydratase, Carbonate Hydrolyase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-576-6
MDL number:
Product Name
Carbonic Anhydrase from bovine erythrocytes, non-denaturing PAGE marker
form
lyophilized powder
mol wt
~29 kDa
suitability
suitable for marker for electrophoresis (non-denaturing PAGE)
storage temp.
−20°C
Quality Level
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Application
This product is used as a marker to determine the molecular mass of an unknown protein in non-denaturing electrophoresis systems. In such systems, proteins often retain their biological activity, thus allowing in-gel detection using specific substrate stains. This technique also facilitates the recovery of the active protein. The product yields 3 bands.
Preparation Note
0.9-1.4 mg/mL after reconstitution with 1 mL of water
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dina Schütze et al.
Journal of cancer research and clinical oncology, 139(5), 747-754 (2013-01-30)
Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies
Frédéric D Birkhäuser et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 36(2), 102-111 (2013-02-05)
The dendritic cell vaccine DC-Ad-GM·CAIX is an active, specific immunotherapy with the potential of providing a safe and effective therapy against renal cell carcinoma (RCC). Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to
Wolfgang Maret
Advances in nutrition (Bethesda, Md.), 4(1), 82-91 (2013-01-16)
The nutritional essentiality of zinc for the growth of living organisms had been recognized long before zinc biochemistry began with the discovery of zinc in carbonic anhydrase in 1939. Painstaking analytical work then demonstrated the presence of zinc as a
Preethi Ragunathan et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 69(Pt 2), 162-164 (2013-02-07)
GK2848, a hypothetical protein from the thermophilic organism Geobacillus kaustophilus, was cloned and overexpressed in Escherichia coli. The protein was purified to homogeneity using Ni-NTA affinity-column and gel-filtration chromatography. The purified protein was crystallized using the sitting-drop vapour-diffusion method. The
Barend Andre Stander et al.
PloS one, 7(12), e52205-e52205 (2013-01-10)
Antimitotic compounds are still one of the most widely used chemotherapeutic anticancer drugs in the clinic today. Given their effectiveness against cancer it is beneficial to continue enhancing these drugs. One way is to improve the bioavailability and efficacy by
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