C8696
Cathepsin D from human liver
lyophilized powder, ≥250 units/mg protein (E1%/280)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥250 units/mg protein (E1%/280)
form
lyophilized powder
specific activity
≥250 units/mg protein (E1%/280)
mol wt
~45 kDa
color
white
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... CTSD(1509)
General description
Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.
Application
Cathepsin D from human liver has been used:
- in β-secretase activity assay
- for enzymatic degradation of amyloid β 1-42
- in microinjection of human foreskin fibroblasts
Biochem/physiol Actions
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts.
Physical form
Powder containing sodium phosphate buffer salt.
Other Notes
Contains α, β and γ isoenzymes as observed by isoelectric-focusing.
One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Platelet membrane beta-secretase activity in mild cognitive impairment and conversion to dementia: a longitudinal study
McGuinness B, et al.
Journal of Alzheimer'S Disease, 49(4), 1095-1103 (2016)
Aspartic Proteinases Physiology and Pathology (1995)
Jeane do Nascimento Moraes et al.
The journal of venomous animals and toxins including tropical diseases, 28, e20220002-e20220002 (2022-11-22)
Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. This protease is a multifunctional enzyme responsible for essential biological processes such as cell cycle regulation, differentiation, migration, tissue remodeling, neuronal growth, ovulation, and apoptosis.
Yoko Shiba et al.
Current biology : CB, 23(19), 1945-1951 (2013-10-01)
ArfGAPs are known to be involved in cargo sorting in COPI transport. However, the role of ArfGAPs in post-Golgi membrane traffic has not been defined. To determine the function of ArfGAPs in post-Golgi traffic, we used small interfering RNA to
Maria Pernemalm et al.
Journal of proteome research, 12(9), 3934-3943 (2013-08-02)
In this study, we have analyzed human primary lung adenocarcinoma tumors using global mass spectrometry to elucidate the biological mechanisms behind relapse post surgery. In total, we identified over 3000 proteins with high confidence. Supervised multivariate analysis was used to
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