D5905
3,3′-Diaminobenzidine tetrahydrochloride
peroxidase substrate, chromogenic, tablet
Synonym(s):
3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB
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About This Item
Linear Formula:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Molecular Weight:
360.11
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:
Beilstein/REAXYS Number:
1212988
Product Name
3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet
Quality Level
form
tablet
solubility
water: 1 tablet/15 mL (may contain small insoluble particles)
storage temp.
−20°C
SMILES string
Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
InChI key
KJDSORYAHBAGPP-UHFFFAOYSA-N
Application
Reagent for spectrophotometric determination of selenium.
3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
Biochem/physiol Actions
Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.
3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.
Preparation Note
Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
ppe
Eyeshields, Gloves, type N95 (US)
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