DNTP100A
Deoxynucleotide Set, 100 mM
Individual dNTPs for routine PCR; 1 mL each
Synonym(s):
PCR dNTPs, dNTP set, dNTPs
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106305
Form:
liquid
Storage temp.:
−20°C
Quality Level
form
liquid
concentration
100 mM
technique(s)
PCR: suitable
color
colorless
shipped in
dry ice
storage temp.
−20°C
General description
Deoxynucleotide triphosphates (dNTPs) are negatively charged organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 1.0 mL each of 100mM aqueous solutions of four deoxyribonucleotides namely deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.
Application
Deoxynucleotide set, 100 mM has been used in:
- the amplification of DNA in polymerase chain reaction (PCR)
- quantitative PCR (qPCR)
- multiplex PCR
Biochem/physiol Actions
Deoxynucleotide triphosphates (dNTPs) are building blocks of nucleic acid molecules and are acidic in nature. They act as substrates for DNA polymerizing enzymes. dNTPs are essential components of PCR master mixes as it is necessary for DNA amplification reaction. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.
Features and Benefits
- Provided as 4 separate tubes of dNTPs
- Allows optimization of individual dNTP concentrations
Packaging
1.0 mL each of 100 mM dATP, dCTP, dGTP and dTTP
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Presicce GA, et al.
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A critical balance: dNTPs and the maintenance of genome stability
Pai CC and Kearsey SE
Gene, 8(2), 57-57 (2017)