M5519
Murashige and Skoog Basal Medium
powder, suitable for plant cell culture
Synonym(s):
MS0 Medium, MSO Medium, MS Basal Medium
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
Quality Level
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General description
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Application
Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.
Preparation Note
Formulated to contain 4.4 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Youjun Zhang et al.
Plant communications, 1(5), 100028-100028 (2020-12-29)
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and
Thierry G A Lonhienne et al.
Scientific reports, 4, 5055-5055 (2014-05-24)
Growth, morphogenesis and function of roots are influenced by the concentration and form of nutrients present in soils, including low molecular mass inorganic N (IN, ammonium, nitrate) and organic N (ON, e.g. amino acids). Proteins, ON of high molecular mass
Sina Fischer et al.
Journal of experimental botany, 72(2), 415-425 (2020-10-11)
High Arsenic Concentration 1 (HAC1), an Arabidopsis thaliana arsenate reductase, plays a key role in arsenate [As(V)] tolerance. Through conversion of As(V) to arsenite [As(III)], HAC1 enables As(III) export from roots, and restricts translocation of As(V) to shoots. To probe
Jingyi Wang et al.
Scientific reports, 9(1), 141-141 (2019-01-16)
Abiotic stress significantly impacts growth and yield of crop plants. It is imperative for crop improvement to discover and utilize stress-tolerant functional genes. In this study, genes responding to abiotic stresses, such as freezing, salt and osmotic stress, were screened
Amr R A Kataya et al.
PloS one, 12(7), e0180478-e0180478 (2017-07-06)
Eukaryotic protein phosphatase 4 (PP4) is a PP2A-type protein phosphatase that is part of a conserved complex with regulatory factors PSY2 and PP4R2. Various lines of Arabidopsis thaliana with mutated PP4 subunit genes were constructed to study the so far
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