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Merck

N3755

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym(s):

Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease

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About This Item

CAS Number:
9013-53-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-748-0
MDL number:
MFCD00131731
EC Number:
3.1.31.1 (BRENDA, IUBMB)
Specific activity:
100-300 units/mg protein
Biological source:
Staphylococcus aureus

Key Documents

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Product Name

Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein

biological source

Staphylococcus aureus

form

lyophilized powder

specific activity

100-300 units/mg protein

technique(s)

DNA purification: suitable

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Quality Level

300

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.
One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000476833
0000476832
0000476834
0000476833
0000476832

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C Dingwall et al.
Nucleic acids research, 9(12), 2659-2673 (1981-06-25)
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
Julien Roche et al.
Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)
The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)
Oliver Rothfuss et al.
Human molecular genetics, 18(20), 3832-3850 (2009-07-21)
Mutations in the parkin gene are the most common cause of recessive familial Parkinson disease (PD). Parkin has been initially characterized as an ubiquitin E3 ligase, but the pathological relevance of this activity remains uncertain. Recently, an impressive amount of
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