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Merck

P4524

β-Lactamase from Enterobacter cloacae

Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

Synonym(s):

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

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About This Item

CAS Number:
9073-60-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.5.2.6 (BRENDA, IUBMB)
MDL number:
MFCD00131819
Specific activity:
0.2-0.6 units/mg protein (using benzylpenicillin)
Biological source:
bacterial (Enterobacter cloacae)

Key Documents

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biological source

bacterial (Enterobacter cloacae)

type

Type IV

form

lyophilized powder

specific activity

0.2-0.6 units/mg protein (using benzylpenicillin)

mol wt

29 kDa

composition

Protein, ~60%

technique(s)

enzyme immunoassay: suitable

suitability

suitable for enzyme test

application(s)

pharmaceutical

storage temp.

2-8°C

Quality Level

200

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Analysis Note

Protein determined by biuret.

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.
β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

General description

β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.

Other Notes

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing sodium phosphate buffer salts

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000430625
0000216044
0000430625
0000430624
0000430624

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Past and present perspectives on beta$-lactamases
Bush K
Antimicrobial Agents and Chemotherapy, 62(10) (2018)
Persistent bacteremia in rabbit fetuses despite maternal antibiotic therapy in a novel intrauterine-infection model
Gras-Le Guen C, et al.
Antimicrobial Agents and Chemotherapy, 47(7), 2125-2130 (2003)
Protein-protein interactions monitored in mammalian cells via complementation of beta-lactamase enzyme fragments
Wehrman T, et al.
Proceedings of the National Academy of Sciences of the USA, 99(6), 3469-3474 (2002)
Jung-Ho Shin et al.
EMBO reports, 22(2), e51790-e51790 (2021-01-20)
Bactericidal antibiotics are powerful agents due to their ability to convert essential bacterial functions into lethal processes. However, many important bacterial pathogens are remarkably tolerant against bactericidal antibiotics due to inducible damage repair responses. The cell wall damage response two-component
P J Johnsen et al.
Genetics, 181(4), 1521-1533 (2009-02-05)
We present a new hypothesis for the selective pressures responsible for maintaining natural competence and transformation. Our hypothesis is based in part on the observation that in Bacillus subtilis, where transformation is widespread, competence is associated with periods of nongrowth
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