P6001
p-Phenylenediamine
98% (GC)
Synonym(s):
1,4-Benzenediamine, 1,4-Diaminobenzene, 1,4-Phenylenediamine
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About This Item
Product Name
p-Phenylenediamine, 98% (GC)
InChI
1S/C6H8N2/c7-5-1-2-6(8)4-3-5/h1-4H,7-8H2
InChI key
CBCKQZAAMUWICA-UHFFFAOYSA-N
SMILES string
Nc1ccc(N)cc1
vapor density
3.7 (vs air)
vapor pressure
1.08 mmHg ( 100 °C)
description
anti-fade reagent
assay
98% (GC)
bp
267 °C (lit.)
mp
138-143 °C (lit.)
Quality Level
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Related Categories
Application
- to evaluate its sensitization potential in vitro in langerhans-like dendritic cells and autologous T cells
- to treat the dorsal sides of the ears of mice, to study its systemic immunological effects
- in glycerol to coverslip coronal sections in the attempt to count Aβ plaques
Anti-fade reagent is added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.
Biochem/physiol Actions
Disclaimer
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 1 Oral
target_organs
Kidney,Heart,Musculo-skeletal system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
230.0 °F - closed cup
flash_point_c
110 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility of p-Phenylediamine, Product P6001?
This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.
For what applications is p-Phenylediamine, Product P6001, used?
This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.
What is the working concentration for p-Phenylediamine, Product P6001, when used as an antifade agent?
J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)".
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