T7791
Thymidine 5′-triphosphate sodium salt solution
10 mM (pH 7.0)
Synonym(s):
TTP
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About This Item
CAS Number:
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥99%
Form:
liquid
Storage temp.:
−20°C
assay
≥99%
Quality Level
form
liquid
feature
PCR Additives
concentration
10 mM (pH 7.0)
technique(s)
PCR: suitable
color
colorless
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
SMILES string
[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
InChI
1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key
KAELIXTVSANDSE-UHFFFAOYSA-N
General description
Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
Application
Thymidine 5′-triphosphate sodium salt solution has been used:
- as a standard to quantify each dNTP from cellular lysates by the liquid chromatography-mass spectrometry (LC-MS/MS) method
- as a component of the dNTP mix for labeling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe
- for routine PCR amplifications
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Xinhui Chen et al.
Biomedical chromatography : BMC, 31(3) (2016-08-25)
The endogenous deoxynucleoside triphosphate (dNTP) pool includes deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and thymidine triphosphate (TTP). The endogenous dNTP pool is regulated by complex enzymatic pathways that can be targeted by drugs, such as antimetabolites. In
Michael G Mohsen et al.
Nucleic acids research, 47(18), 9495-9501 (2019-09-11)
We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the