581513-U
Ascentis® Si (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(s):
Silica-Based HPLC Column
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About This Item
eCl@ss:
32110501
UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
silica phase
Pore size:
100 Å
Matrix:
fully porous particle, silica gel high purity, spherical
Product Name
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L3
product line
Ascentis®
feature
endcapped: no
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
impurities
<5 ppm metals
matrix
fully porous particle, silica gel high purity, spherical
matrix active group
silica phase
particle size
5 μm
pore size
100 Å
operating pH range
2-6
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC), normal phase
General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Anna Różańska et al.
Foods (Basel, Switzerland), 9(8) (2020-08-23)
High quality extra virgin olive oils represent an optimal source of nutraceuticals. The European Union (EU) is the world's leading olive oil producer, with the Mediterranean region as the main contributor. This makes the EU the greatest exporter and consumer
Deborah Benhaim et al.
Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)
This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities