59354-U
Discovery® C8 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(s):
C8 Reversed-Phase Chromatography Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C8 (RP8), C8 (octyl) phase
Pore size:
180 Å
Matrix:
silica gel, high purity, spherical base material, fully porous particle
Product Name
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Segment
agency
suitable for USP L7
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
7.5% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
200 m2/g
surface coverage
3.4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical base material, fully porous particle
matrix active group
C8 (RP8), C8 (octyl) phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Application
Discovery® C8 HPLC column may be used in the determination of bisphenol A, genistein, bisphenol A β-D-glucuronide (BPA gluc), and genistein 4′-β-D-glucuronide (genistein gluc) in biological matrices including urine, cultures and tissues using solid-phase microextraction (SPME), followed by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS).
Features and Benefits
- Excellent reproducibility
- Faster separation of strongly hydrophobic analytes than C18 columns
- Stable, low-bleed LC-MS separations
- Exceptional peak shapes for basic and acidic compounds
- Compatible with low organic/highly aqueous mobile phases
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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