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AP1179 Anti-TLR4 Mouse mAb (1H7)

This Anti-TLR4 Mouse mAb (1H7) is validated for use in ELISA, Immunoblotting for the detection of TLR4.

Anti-TLR4 Mouse mAb (1H7) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Toll-like Receptor 4

AP1179
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

RéférenceConditionnement Qté
AP1179-100UG 100 μg
Description
OverviewRecognizes the TLR4 protein.
Catalogue NumberAP1179
Brand Family Calbiochem®
SynonymsAnti-Toll-like Receptor 4
Application Data
Detection of human TLR4 by immunoblotting. Sample: Partial recombinant TLR4. Primary antibody: Anti-TLR4 Mouse mAb (1H7) (Cat. No. AP1179) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 214-292 human TLR4, expressed as a GST fusion protein
Clone1H7
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
AP1179-100UG 04055977212723

Documentation

Anti-TLR4 Mouse mAb (1H7) FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-TLR4 Mouse mAb (1H7) Certificats d'analyse

TitreNuméro de lot
AP1179
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-Toll-like Receptor 4
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human TLR4 by immunoblotting. Sample: Partial recombinant TLR4. Primary antibody: Anti-TLR4 Mouse mAb (1H7) (Cat. No. AP1179) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the TLR4 protein.
BackgroundTLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 214-292 human TLR4, expressed as a GST fusion protein
Clone1H7
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review