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Merck

251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

Synonym(s):

DAR-4M AM, Diaminorhodamine-4M AM

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About This Item

Empirical Formula (Hill Notation):
C28H31IN4O5
Molecular Weight:
630.47
NACRES:
NA.25
UNSPSC Code:
12352202

Product Name

DAR-4M AM, A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

dark red

fluorescence

λex ~560 nm
λem ~575 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Irritant (B)

General description

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

Legal Information

Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Packaging

Packaged under inert gas

Physical form

In DMSO.

Preparation Note

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C

Certificates of Analysis (COA)

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