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EDS

Ethylenediaminetetraacetic acid

Name: Ethylenediaminetetraacetic acid
Brand: SIAL

BioUltra, anhydrous, ≥99% (titration)

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

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About This Item

Linear Formula:

(HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂

CAS Number:

60-00-4

Molecular Weight:

292.24

NACRES:

NA.77

PubChem Substance ID:

24894713

eCl@ss:

39030907

UNSPSC Code:

12352116

EC Number:

200-449-4

MDL number:

MFCD00003541

Beilstein/REAXYS Number:

1716295

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Properties

grade

anhydrous

Quality Segment

200

product line

BioUltra

assay

≥99% (titration)

form

powder

reaction suitability

reagent type: chelator

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is a substituted diamine and a versatile compound in household with industrial applications. EDTA chelates metal divalent cations like calcium, magnesium, zinc, copper, manganese by forming metal-EDTA complexes. It finds its application in cleaning products and detergent formulations. In paper and pulp industries, EDTA reduces the adverse effect of metal ions on bleaching. It functions as an antibacterial agent by removing calcium and magnesium divalent cations from the outer membrane and causes loss of lipopolysaccharide from the membrane, making the bacteria susceptible to bactericidal agents. Free EDTA has adverse effect on reproduction and development in mammals. EDTA can chelate ions from deposits that contribute to atherosclerosis, cancers and heart diseases. It can help to overcome cell membrane injury by reducing radical reactions and oxidation processes. It binds to calcium and reduces the risk of hypercalcinemia in cancer patients. Transmembrane ammonium EDTA gradient method aids in drug delivery, increasing drug retention and efficacy of therapeutics and lowers the cytotoxicity levels.

Application

EDTA has been used:

as a medium supplement in embryo culture using synthetic oviduct fluid culture 1 (SOFC1) medium
in incubation of EpCAM+ cells with fluorescein isothiocyanate (FITC) conjugated antibody for cell sorting analysis
in pre-treatment of single cell suspension of dendritic cells from spleen for cell sorting with magnetic beads

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Signal Word

Warning

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

Target Organs

Respiratory Tract

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - STOT RE 2 Inhalation

WGK

WGK 3

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