BIS-TRIS propane

Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments.

Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).

BIS-TRIS Propane may be used in the following studies:

• as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO₂).
• for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.
• as a buffer during GeO₂ mineralisation.
• as a buffer component in a study pertaining to environmental research
• as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes

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