567507-U
Discovery® HS F5 (3 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
Discovery Pentafluorophenyl PFP HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
15 cm × 4.6 mm
Particle size:
3 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
120 Å
Matrix:
silica gel, high purity, spherical particle platform, fully porous particle
Product Name
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Quality Segment
agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform, fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
3 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Application
- Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis.: This study uses the Discovery® HS F5 HPLC Column to identify and quantify the main contaminant in primaquine diphosphate, crucial for ensuring the quality and safety of this antimalarial drug (Brondz et al., 2007).
- HPLC assisted chemobiological standardization of alpha-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant.: Demonstrates the application of the Discovery® HS F5 HPLC Column in the biologically relevant chemical analysis of Indian medicinal plants, contributing to the standardization of herbal medicine (Pullela et al., 2006).
- Disposition study of a new potential antineoplastic agent dimefluron in rats using high-performance liquid chromatography with ultraviolet and mass spectrometric detection.: Utilizes the Discovery® HS F5 HPLC Column to study the pharmacokinetics and metabolism of a novel anticancer agent, aiding in the development of effective cancer therapies (Císar et al., 2005).
- A simplified analytical method for a phenotyping cocktail of major CYP450 biotransformation routes.: This research employs the Discovery® HS F5 HPLC Column to simplify the analysis of a phenotyping cocktail, enhancing the assessment of drug metabolism and facilitating personalized medicine approaches (Jerdi et al., 2004).
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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