59354-U
Discovery® C8 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(s):
C8 Reversed-Phase Chromatography Column
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C8 (octyl) phase
Pore size:
180 Å
Matrix:
silica gel, high purity, spherical base material, fully porous particle
Product Name
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L7
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
7.5% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
200 m2/g
surface coverage
3.4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical base material, fully porous particle
matrix active group
C8 (octyl) phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Application
Discovery® C8 HPLC column may be used in the determination of bisphenol A, genistein, bisphenol A β-D-glucuronide (BPA gluc), and genistein 4′-β-D-glucuronide (genistein gluc) in biological matrices including urine, cultures and tissues using solid-phase microextraction (SPME), followed by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS).
Features and Benefits
- Excellent reproducibility
- Faster separation of strongly hydrophobic analytes than C18 columns
- Stable, low-bleed LC-MS separations
- Exceptional peak shapes for basic and acidic compounds
- Compatible with low organic/highly aqueous mobile phases
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Explore all of our products under Discovery® C8 HPLC Column
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
General Care and Use of Supelco™ HPLC Columns
Measurement of bisphenol A, bisphenol A β-d-glucuronide, genistein, and genistein 4′- β-d-glucuronide via SPE and HPLC-MS/MS.
Coughlin LJ, et al.
Analytical and Bioanalytical Chemistry, 401, 995-1002 (2011)
Rahul Palchaudhuri et al.
Cell reports, 13(9), 2027-2036 (2015-12-15)
Apoptosis is generally believed to be a process that requires several hours, in contrast to non-programmed forms of cell death that can occur in minutes. Our findings challenge the time-consuming nature of apoptosis as we describe the discovery and characterization
Laurence Conraux et al.
PloS one, 8(11), e79733-e79733 (2013-11-14)
The diagnostic of Amyotrophic lateral sclerosis (ALS) remains based on clinical and neurophysiological observations. The actual delay between the onset of the symptoms and diagnosis is about 1 year, preventing early inclusion of patients into clinical trials and early care