P1944
Phenol:Chloroform:Iso-amyl alcohol (125:24:1)
Molecular Biology
Synonym(s):
phenol chloroform RNA extraction
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About This Item
NACRES:
NA.52
UNSPSC Code:
12190000
Assay:
≥99.0%
Grade:
Molecular Biology
grade
Molecular Biology
Quality Level
assay
≥99.0%
form
liquid
pH
4.3-4.7
application(s)
life science and biopharma
storage temp.
2-8°C
General description
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.
Application
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
- for the extraction and purification of RNA.
- to isolate RNA from Arabidopsis siliques.
- to extract proteins and DNA from polysomal and non-polysomal fractions.
- in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
target_organs
Liver,Kidney, Nervous system,Kidney,Liver,Skin
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
175.0 °F - closed cup
flash_point_c
79.44 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Datasheet
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)