D0531
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride
≥90% purity (HPLC), powder
Synonym(s):
DTAF
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About This Item
Empirical Formula (Hill Notation):
C23H12Cl2N4O5 · HCl
CAS Number:
Molecular Weight:
531.73
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
Product Name
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride, ≥90% (HPLC)
Quality Level
assay
≥90% (HPLC)
form
powder
color
yellow to orange
fluorescence
λex 495 nm; λem 516 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1
InChI
1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H
InChI key
CEKSHCAIHGNWBK-UHFFFAOYSA-N
Application
A fluorescein derivative which reacts with amino groups and N-terminal amino acids of proteins at neutral pH.
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea). It has also been used to label bacteria (Vibrio spp.).
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Synergistic gelation of gelatin B with xanthan gum
Wang CS, et al.
Food Hydrocolloids, 60, 374-383 (2016)
Adrián Pérez-Ramos et al.
Frontiers in microbiology, 9, 1684-1684 (2018-08-10)
Lactic acid bacteria synthesize exopolysaccharides (EPS), which could benefit the host's health as immunomodulators. Furthermore, EPS could protect bacteria against gastrointestinal stress, favoring gut colonization, thus protecting the host against pathogenic infections. Pediococcus parvulus 2.6, produces a 2-substituted (1,3)-β-D-glucan and
Assessment of fluorescent-labeled bacteria for evaluation of in vivo uptake of bacteria (Vibrio spp.) by crustacean larvae.
Soto-Rodriguez SA, et al.
Journal of Microbiological Methods, 52, 101-114 (2003)