49525
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt
≥99.0% (TLC), gamma-glutamyltransferase substrate, powder
Synonym(s):
L-Glutamic acid 5-(3-carboxy-4-nitroanilide) ammonium salt
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About This Item
Linear Formula:
C12H12N3O7NH4
CAS Number:
Molecular Weight:
328.28
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
264-418-7
MDL number:
Product Name
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt, ≥99.0% (TLC)
Quality Level
assay
≥99.0% (TLC)
form
powder
impurities
≤6% water
solubility
H2O: 100 mg/mL, clear, yellow-green
suitability
suitable as substrate for γ-glutamyltranspeptidase test
storage temp.
2-8°C
SMILES string
N.N[C@@H](CCC(=O)Nc1ccc(c(c1)C(O)=O)[N+]([O-])=O)C(O)=O
InChI
1S/C12H13N3O7.H3N/c13-8(12(19)20)2-4-10(16)14-6-1-3-9(15(21)22)7(5-6)11(17)18;/h1,3,5,8H,2,4,13H2,(H,14,16)(H,17,18)(H,19,20);1H3/t8-;/m0./s1
InChI key
GFABNNMTRVBLPZ-QRPNPIFTSA-N
General description
Substrate for γ-glutamyl transferase.
Application
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt has been used as a synthetic substrate for gamma-glutamyltransferase (GGT) to determine GGT activity.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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L M Shaw et al.
Clinical chemistry, 23(1), 79-85 (1977-01-01)
The kinetics of human serum gamma-glutamyltransferase (EC 2.3.2.2) were investigated, with use of glycylglycine as a gamma-glutamyl acceptor substrate and gamma-glutamyl-4-nitroanilide and its carboxy derivative, gamma-glutamyl-3-carboxy-4-nitroanilide, as donor substrates. The simultaneous occurrence of both gamma-glutamyltransfer and autotransfer was established by
A kinetic study of gamma-glutamyltransferase (GGT)-mediated S-nitrosoglutathione catabolism.
Angeli V
Archives of Biochemistry and Biophysics, 481(2), 191-196 (2009)