A5043
Adenosine Deaminase from bovine spleen
Type X, buffered aqueous glycerol solution, ≥130 units/mg protein
Synonym(s):
ADA, adenosine aminohydrolase, Adenosine aminohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥130 units/mg protein
Biological source:
bovine spleen
biological source
bovine spleen
Quality Level
type
Type X
form
buffered aqueous glycerol solution
specific activity
≥130 units/mg protein
mol wt
32.5-33 kDa
cation traces
NH4+: ≤10 μg/mg protein
UniProt accession no.
foreign activity
alkaline phosphatase ≤0.01%, guanase ≤0.01%, nucleoside phosphorylase ≤0.1%
storage temp.
2-8°C
Gene Information
cow ... ADA(280712)
General description
Molecular weight: 32.5-33 kDa
pI: 4.85
pI: 4.85
Application
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Biochem/physiol Actions
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Physical form
Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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