C0528
Casein fluorescein isothiocyanate from bovine milk
Type III, essentially salt-free, lyophilized powder
Synonym(s):
FITC-casein
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About This Item
type
Type III
Quality Level
form
essentially salt-free, lyophilized powder
extent of labeling
≥50 μg FITC per mg solid
solubility
water: 5 mg/mL, clear to hazy
storage temp.
2-8°C
Application
highly sensitive protease substrate
Casein fluorescein isothiocyanate from bovine milk has been used:
- in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment
- to make a reference line in immunochromatographic devices
- in running gel for the documentation of proteinases
Biochem/physiol Actions
Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The supplementation of culture medium with protease improves the hatching rate of mouse embryos
Lee DR, et al.
Human Reproduction, 12(11), 2493-2498 (1997)
Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Casein, fluorescein isothiocyanate as a substrate for measuring protease production by psychrotrophic bacteria
Christen GL and Senica M
Journal of Food Protection, 50(9), 744-749 (1987)