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Merck

63069

Magnesium chloride solution

BioUltra, Molecular Biology, ~1 M in H2O

Synonym(s):

Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2

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About This Item

Linear Formula:
MgCl2
CAS Number:
7786-30-3
Molecular Weight:
95.21
NACRES:
NA.26
PubChem Substance ID:
329759772
UNSPSC Code:
12352302
MDL number:
MFCD00011106
grade:
Molecular Biology
form:
liquid
solubility:
water: soluble

Product Name

Magnesium chloride solution, BioUltra, Molecular Biology, ~1 M in H2O

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

SMILES string

Cl[Mg]Cl

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

grade

Molecular Biology

product line

BioUltra

form

liquid

concentration

~1 M in H2O

technique(s)

HPLC: suitable
flow cytometry: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.0002% total Nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

water: soluble

density

1.08 g/mL at 20 °C

anion traces

phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

suitability

suitable for molecular biology

application(s)

cell analysis
filtration
vaccine development

Quality Level

200

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Application

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).
Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

General description

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.

Other Notes

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1453
BCCD4296
BCCD4298
BCCM3946
BCCJ0554

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