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Merck

46951

Fluorescein isothiocyanate isomer I

≥97.5% (HPLC)

Synonym(s):

FITC, Fluorescein 5-isothiocyanate

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
EC Number:
222-042-0
UNSPSC Code:
12171500
PubChem Substance ID:
329757274
Beilstein/REAXYS Number:
1407295
MDL number:
MFCD00005063

Product Name

Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

assay

≥97.5% (HPLC)

form

powder

impurities

≤1.5% Isomer II

mp

>360 °C (lit.)

solubility

acetone: soluble 0.1 g/10 mL

fluorescence

λex 492 nm; λem 518 nm (green)
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

storage temp.

2-8°C

Quality Level

100

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Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCBK0797V

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