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94280

Urease from Canavalia ensiformis (Jack bean)

powder, ~1 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:
9002-13-5
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
MFCD00070858
EC Number:
3.5.1.5 (BRENDA, IUBMB)
Specific activity:
~1 U/mg
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form

powder

Quality Segment

100

specific activity

~1 U/mg

mol wt

Mr ~480000

storage temp.

−20°C

General description

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.
Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.
Application in (selective) hydrolysis/condensations;citation structure.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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