A2064

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal

Name: Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal
Brand: SIGMA

clone GG-5, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgG (γ-chain specific)

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About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Product Name

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal, clone GG-5, purified from hybridoma cell culture

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

GG-5, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry: suitable
western blot (chemiluminescent): 1:80,000

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

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Related Categories

Protein Biology

Secondary Antibodies

Antibodies - Primary, Secondary & Recombinant Monoclonal

Application

Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:

enzyme linked immunosorbent assay (ELISA)
western blotting
dot blot
immunohistology

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

Liquid precipitate (Lppt).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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An acquired, calcium-dependent, factor X inhibitor.

George J Broze

Blood cells, molecules & diseases, 52(2-3), 116-120 (2013-10-01)

Acquired factor X (FX) deficiency unrelated to amyloidosis is a rare disorder in which an anti-FX antibody is infrequently detected. A patient with severe bleeding due to a calcium ion-dependent anti-FX IgG antibody is described. The FX affinity purified IgG

Safety and Immunogenicity of a Pseudomonas aeruginosa Hybrid Outer Membrane Protein F-I Vaccine in Human Volunteers

Mansouri E, et al.

Infection and Immunity, 67(7), 1461-1470 (1999)

Amorphous Ca²⁺ polyphosphate nanoparticles regulate the ATP level in bone-like SaOS-2 cells.

Werner E G Müller et al.

Journal of cell science, 128(11), 2202-2207 (2015-04-25)

Polyphosphate (polyP) is a physiologically occurring polyanion that is synthesized especially in bone-forming osteoblast cells and blood platelets. We used amorphous polyP nanoparticles, complexed with Ca(2+), that have a globular size of ∼100 nm. Because polyP comprises inorganic orthophosphate units

Receptor-mediated targeting of spray-dried lipid particles coformulated with immunoglobulin and loaded with a prototype vaccine

Bot AI, et al.

Pharmaceutical Research, 18(7), 971-979 (2001)

Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity.

Camila A Wilkens et al.

PloS one, 10(3), e0119053-e0119053 (2015-03-15)

Cell engineering has been used to improve animal cells' central carbon metabolism. Due to the central carbon metabolism's inefficiency and limiting input of carbons into the TCA cycle, key reactions belonging to these pathways have been targeted to improve cultures'

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