A6014

Acridine Orange hemi(zinc chloride) salt

Name: Acridine Orange hemi(zinc chloride) salt
Brand: SIGMA

For nucleic acid staining in cells or gels

Synonym(s):

3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14

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About This Item

Linear Formula:

C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂

CAS Number:

10127-02-3

Molecular Weight:

369.96

EC Number:

233-353-6

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

24891048

MDL number:

MFCD00081043

Colour Index Number:

46005

Beilstein/REAXYS Number:

3734978

Product Name

Acridine Orange hemi(zinc chloride) salt, For nucleic acid staining in cells or gels

InChI key

RAHGLSRJKRXOSY-UHFFFAOYSA-L

InChI

1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2

SMILES string

Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C

product line

BioReagent

form

powder

composition

Dye content, ~80%

technique(s)

nucleic acid detection: suitable

solubility

ethanol: 2 mg/mL
4 mg/mL (2-methoxyethanol (EGME))
water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)

suitability

suitable for flow cytometry
suitable for microscopy

storage temp.

room temp

Quality Level

200

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Application

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Suitable for

detection of nucleic acids separated by gel electrophoresis
fluorescence and epifluorescence microscopy
analysis of mitochondria and lysosomes by flow cytometry
DNA staining in apoptosis studies

Biochem/physiol Actions

Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.

Features and Benefits

120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
differential staining of single- and double-stranded polynucleotides

General description

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

pictograms

GHS08

signalword

Warning

hcodes

H341

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Muta. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Features of apoptotic cells measured by flow cytometry.

Z Darzynkiewicz et al.

Cytometry, 13(8), 795-808 (1992-01-01)

The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

G K McMaster et al.

Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)

We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The

Quick visualization of neurons in brain tissues using an optical clearing technique.

Yu Sato et al.

Anatomical science international, 94(2), 199-208 (2019-01-03)

Neurons are classified into several morphological types according to the locations of their somata and the branching patterns of their axons and dendrites. Recent studies suggest that these morphological features are related to their physiological properties, including firing characteristics, responses

[Lysosome changes in exponentially growing, synchronized and differentiating L-cell cultures].

A B Borisov et al.

Tsitologiia, 24(10), 1233-1237 (1982-10-01)

Using supravital fluorescent staining of lysosomes with Euchrysine 3R, the morphology of these organelles was studied in L cells examined from cultures being at different growth phases in the course of cell cycle and after adipocyte conversion of L cells

Degradation of apoptotic cells and fragments in HL-60 suspension cultures after induction of apoptosis by camptothecin and ethanol.

H Baisch et al.

Cell proliferation, 32(5), 303-319 (2000-01-05)

Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of

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