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Merck

A9655

Auramine O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
Molecular Weight:
303.83
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
219-567-2
MDL number:
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061
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Product Name

Auramine O, Dye content ≥80 %, certified by the BSC

agency

certified by the BSC

Quality Level

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O, 432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).


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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

wgk

WGK 3

Storage Class

11 - Combustible Solids



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Related Content

Product Information Sheet


Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).
Inbaraj B S, et al.
Biochemical Engineering Journal, 31(3), 204-215 (2006)
E W Koneman.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)
S Wilson et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(9), 1164-1168 (2010-09-08)
Direct sputum smear microscopy is usually less sensitive than the indirect approach using concentration by centrifugation, but laboratories often do not have access to appropriate equipment. An alternative method of sample concentration has been developed based on magnetic beads coated