C4018
Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)
lyophilized powder, ≥100 units/mg protein
Synonym(s):
3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
form
lyophilized powder
Quality Level
specific activity
≥100 units/mg protein
composition
Protein, ≥50% biuret
shipped in
wet ice
storage temp.
−20°C
Application
The enzyme from Sigma has been used in the analysis of bile samples in various studies.
Biochem/physiol Actions
The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Other Notes
One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Maksim Rossocha et al.
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
Arekal N Roopashri et al.
Applied microbiology and biotechnology, 83(6), 1115-1126 (2009-05-02)
In a changing scenario of food habits being associated with wellness factors through the concepts of probiotics and prebiotics, an attempt has been made to characterize on molecular basis, the desirable benefits associated with natural isolates of lactic acid bacteria
Guohong Wang et al.
Journal of industrial microbiology & biotechnology, 38(8), 985-990 (2010-09-22)
Lactic acid bacteria (LAB) encounter various types of stress during industrial processes and gastrointestinal transit. Catalase (CAT) and bile salt hydrolase (BSH) can protect bacteria from oxidative stress or damage caused by bile salts by decomposing hydrogen peroxide (H(2)O(2)) or
M Victoria Delpino et al.
Archives of microbiology, 191(7), 571-581 (2009-05-14)
The virB genes coding type IV secretion system are necessary for the intracellular survival and replication of Brucella spp. In this study, extracellular proteins from B. abortus 2308 (wild type, WT) and its isogenic virB10 polar mutant were compared. Culture
Christopher Martoni et al.
Applied microbiology and biotechnology, 81(2), 225-233 (2008-08-23)
This is the first study of its kind to screen probiotic lactic acid bacteria for the purpose of microencapsulating a highly bile salt hydrolase (BSH)-active strain. A Lactobacillus reuteri strain and a Bifidobacterium longum strain were isolated as the highest
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